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Here is a selection of antibodies tested using the ProHisto Antibody Amplifier system. Note that, according to the manufacturer, many of these antibodies are not recommended for IHC or must be used at very low dilutions. ProHisto products solve these shortcomings.The ProHisto system always results in even staining, with low background, while providing a huge cost savings to any scientist.
Anti-Acetylated lycine Anti-AHA1


Anti-Acetylated lycine 1:100,000. Stressmarq Biosciences, Inc.
monoclonal Cat# SMC-153.
Recommended dilution for sedentary method: Not determined.
Human GI tissue; paraffin embedded.


Anti-AHA1 1:100,000 (Stressmarq Biosciences, Inc. Cat# SPC-183)
Recommended dilution for sedentary method: Not determined.
Mouse colitis section, paraffin embedded.
Anti-AKT Anti-AKT1


Anti-AKT 1:5000 (Cell Signaling Technologies. Polyclonal, Rabbit cat# 9272). Recommended dilution for sedentary method: 1:50. Mouse colitis section. Paraffin embedded.100X MORE DILUTED THAN SEDENTARY METHOD.

Anti-AKT1 1:10,000 (Genetex, Inc.. Polyclonal, cat# GTX1015574).
 Recommended dilution for sedentary method: Not determined.
 Human kidney cancer section. Paraffin embedded.
Anti-AKT1+2+3 Anti-AKT1+2+3


Anti-AKT1 1:5000 (Genetex, Inc.. Polyclonal, cat# GTX121937).
 Recommended dilution for sedentary method: Not determined.
Human esophageal cancer section. Paraffin embedded.


Anti-AKT1+2+3 1:10000 (Genetex, Inc. polyclonal, cat# GTX121936).
Recommended dilution for sedentary method: Not determined.
 Human kidney cancer section. Paraffin embedded.
Anti-AP endonuclease 1 (APE) Anti-Bax



Anti-AP Endonuclease 1 (APE1) 1:1,000,000 (Novus, Monoclonal,
clone 13B8E5C2).
Recommended dilution for sedentary method: 1:5000.
Mouse colitis section. Paraffin embedded.

200X MORE DILUTED THAN SEDENTARY METHOD


Anti-Bax 1: 3,000 (Cell Signaling, Polyclonal, Cat#2772).
Recommended dilution for sedentary method: Not recommended for IHC.
Mouse colitis section. Paraffin embedded.
Anti-Bcl2 Anti-Calnexin-CT



Anti-Bcl2 1: 5,000 (Cell Signaling, Polyclonal, Cat#2876).
Recommended dilution for sedentary method: Not recommended for IHC.
Mouse colitis section. Paraffin embedded.



Anti-Calnexin-CT 1: 10,000 (Stressmarq Biosciences, Inc.
polyclonal, Cat# SPC-182). Recommended dilution for sedentary method:
Not determined for IHC. Mouse colitis section. Paraffin embedded.
Anti-Calnexin-NT Anti-Calreticulin


Anti-Calnexin-NT 1: 10,000 (Stressmarq Biosciences, Inc
 polyclonal, Cat# SPC-127). Recommended dilution for sedentary method:
 Not determined for IHC. Mouse colitis section. Paraffin embedded.


Anti-Calreticulin 1: 10,000 (Stressmarq Biosciences, Inc.polyclonal, Cat# SPC-122).
Recommended dilution for sedentary method: Not determined for IHC.
Mouse colitis section. Paraffin embedded.
Anti-CD4 Anti-CD74


Anti-CD4 1: 20,000 (Lifespan Biosciences, Monoclonal, clone 2Q1171,
Cat# LS-C4476). Recommended dilution for sedentary method:
Not determined for IHC. Mouse colitis section. Paraffin embedded.



Anti-CD74 1: 10,000 (Stressmarq Biosciences, Inc. polyclonal Cat# SPC-116).
Recommended dilution for sedentary method: Not determined.
Human colon cancer section. Paraffin embedded.
Anti-CD8 Anti-COX2



Anti-CD8 1: 100,000 (Abcam, Rabbit Monoclonal, clone EP1150Y,
Cat# ab52854).
Recommended dilution for sedentary method: 1:250.
 Mouse colitis section. Paraffin embedded.

400X MORE DILUTED THAN SEDENTARY METHOD



Anti-COX2 1: 20,000 (Cayman, Polyclonal, Cat#160126).
 Recommended dilution for sedentary method: 1:400.
Mouse colitis section. Paraffin embedded.

50X MORE DILUTED THAN SEDENTARY METHOD


Anti-Crystallin alpha B Anti-DNA/RNA Damage


Anti-Crystallin alpha B 1: 20,000 (Stressmarq Biosciences, Inc. polyclonal,
Cat# SPC-126).
Recommended dilution for sedentary method: Not determined.
Mouse colitis section. Paraffin embedded.



Anti-DNA/RNA Damage 1: 20,000 (Stressmarq Biosciences, Inc.,
Mouse monoclonal, clone 15A3, cat# SMC-155)
(8-hydroxy-guanosine-BSA and –casein conjugates).
Recommended dilution for sedentary method: Not determined.
Human colon cancer section. Paraffin embedded.
Anti-ER alpha Anti-Fas-L



Anti-ER alpha 1: 20,000 (Neomarkers) polyclonal, rabbit cat# RB-1493-P0).
 Recommended dilution for sedentary method: 1:500.
 Human breast cancer section. Paraffin embedded.

40X MORE DILUTED THAN SEDENTARY METHOD



Anti-Fas-L 2.5ug/ml (Calbiochem, polyclonal, Cat#PC78).
Recommended dilution for sedentary method: Not recommended for IHC.
Mouse thymus section. Paraffin embedded.
Anti-FKBP51



Anti-FKBP51 1:10,000 (Stressmarq Biosciences, Inc.,
monoclonal Cat# SMC-139).
Recommended dilution for sedentary method: Not determined.
Human breast cancer section. Paraffin embedded.
Anti-ER alpha 1:20,00 Neomarkers rabbit polyclonal cat# RB-1493-P0