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Here is a selection of antibodies we have tested using the
Antibody Amplifier™.  Some have never worked, or worked
poorly, using the traditional sedentary ("dropping on") method.
We willadd new antibodies to this site as they are tested.
If your lab has success with a particular antibody using the
Antibody Amplifier, we invite you to send a picture and we
will add it to our site with your reference.
Anti-COX2 1:20,000 (Cayman, Polyclonal,
Cat#160126) Recommended Dilution for
sedentary method: 1:400. Colon section
from mouse with colitis, paraffin embedded.
Anti-Ki67 1:1,000,000 (YES, 1 IN 1
MILLION!!!) (Dako, Monoclonal, MIB-1)
Recommended Dilution for sedentary
method: 1:75. Mouse colon section,
paraffin embedded.
Anti-iNOS 1:5000 (Cayman, Polyclonal,
Cat#160862). Recommended Dilution for
sedentary method: Not stated for IHC.
Colon section from mouse with colitis,
paraffin embedded.
FOR A DEMO, SEE:
DEMONSTRATION
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Anti-AP endonuclease 1 (APE1) 1:5,000,000
(YES, 1 IN 5 MILLION!!) (Novus, Monoclonal,
clone 13B8E5C2) Recommended Dilution for
sedentary method: 1:5000.  Mouse Colon
Section, Paraffin embedded.
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1000 FOLD DILUTION
FROM SEDENTARY
METHOD
Anti-p53 1:1,000,000 (YES, 1 IN 1 MILLION!!)
(Exalpha, Biologicals, Inc. Monoclonal, mouse
clone Pab122, cat# X1494M). Recommended
dilution for sedentary method: 1:1000. Mouse
Colon Section from mouse with colitis.  Paraffin
embedded.
Anti-iNOS 1:10,000 (Res & Diagnostic
Antibodies, Monoclonal Clone 5D5-H7, Cat#
MC-5245).  Recommended Dilution for
sedentary method: 1:50.  Colon section from
mouse with mild colitis, paraffin embedded.
Anti-Phospho-Serine-15 53 1:20,000 (Mouse monoclonal, Cell Signaling,
Clone 16G8, cat#9286S). Recommended dilution for sedentary method:
1:100. A. Human Colon Cancer; B. Mouse Colitis Section.  Paraffin embedded
200 FOLD DILUTION
FROM SEDENTARY
METHOD
CONTACT
Phone: 803-407-0506
Fax: 803-407-0260
Email: prohisto@yahoo.com
Anti-AKT 1:5,000 (Cell Signaling Technologies.
Polyclonal, Rabbit cat# 9272). Recommended
dilution for sedentary method: 1:50. Mouse Colon
Section from normal mouse.  Paraffin embedded.
100 FOLD DILUTION
FROM SEDENTARY
METHOD
1000 FOLD DILUTION
FROM SEDENTARY
METHOD
13,333 FOLD DILUTION
FROM SEDENTARY
METHOD
200 FOLD DILUTION
FROM SEDENTARY
METHOD
PRODUCT INSERT DOES NOT
SAY IT CAN BE USED WITH
PARAFIN EMBEDDED
TISSUE SECTIONS
50 FOLD DILUTION
FROM SEDENTARY
METHOD
Anti P21WAF1 1:2000 (Dako, Monoclonal,
SX108). Recommended Dilution for
sedentary method: 1:25. Mouse Thymus
Section, paraffin embedded.
80 FOLD DILUTION
FROM SEDENTARY
METHOD
Anti-p44/p42 MAP Kinase 1:1,000 (Cell
Signaling Technologies, Polyclonal, Cat#9102).
 Recommended Dilution for sedentary
method: 1:25. Human breast cancer section.
40 FOLD DILUTION
FROM SEDENTARY
METHOD
Anti-ERalpha 1:20,000 (Neomarkers) Polyclonal,
rabbit cat# RB-1493-P0). Recommended dilution
for sedentary method: 1:500. Human Breast
Cancer Section.  Paraffin embedded.
40 FOLD DILUTION
FROM SEDENTARY
METHOD
NEXT
Anti-DNA/RNA Damage 1:10,000 (Stressmarq  
Biosciences, Inc., Mouse Monoclonal, clone
15A3, cat# SMC-155) (8-hydroxy-guanosine-BSA
and –casein conjugates). Recommended
dilution for sedentary method: 1:130. Mouse
Colon Section from mouse with colitis.  Paraffin
embedded.
Anti-Nitrotyrosine 1:10,000 (Stressmarq  
Biosciences, Inc., Mouse Monoclonal, clone
39B6, cat# SMC-154). Recommended dilution
for sedentary method: 1:1000. Mouse Colon
Section from mouse with colitis.  Paraffin
embedded.
100 FOLD DILUTION
FROM SEDENTARY
METHOD
770 FOLD DILUTION
FROM SEDENTARY
METHOD