Here is a selection of antibodies we have tested using the
Antibody Amplifier™. Some have never worked, or worked
poorly, using the traditional "dropping on" method. We will
add new antibodies to this site as they are tested. If your lab
has success with a particular antibody using the Antibody
Amplifier, we invite you to send a picture and we will add
it to our site with your reference.
Antibody Amplifier™. Some have never worked, or worked
poorly, using the traditional "dropping on" method. We will
add new antibodies to this site as they are tested. If your lab
has success with a particular antibody using the Antibody
Amplifier, we invite you to send a picture and we will add
it to our site with your reference.
Anti-COX2 1:20,000 (Cayman,
Polyclonal, Cat#160126)
Company Recommended Dilution:
1:400. Colon section from mouse
with colitis, paraffin embedded.
Polyclonal, Cat#160126)
Company Recommended Dilution:
1:400. Colon section from mouse
with colitis, paraffin embedded.
Anti-Ki67 1:1,000,000 (YES, 1 IN 1
MILLION!!!) (Dako, Monoclonal, MIB-1)
Company Recommended Dilution:
1:75. Mouse colon section, paraffin
embedded.
MILLION!!!) (Dako, Monoclonal, MIB-1)
Company Recommended Dilution:
1:75. Mouse colon section, paraffin
embedded.
Anti-iNOS 1:5000 (Cayman, Polyclonal,
Cat#160862). Company Recommended
Dilution: Not stated for IHC. Colon
section from mouse with colitis, paraffin
embedded.
Cat#160862). Company Recommended
Dilution: Not stated for IHC. Colon
section from mouse with colitis, paraffin
embedded.
FOR A DEMO, SEE:
DEMONSTRATION
DEMONSTRATION
Anti-AP endonuclease 1 (APE1) 1:5,000,000
(YES, 1 IN 5 MILLION!!) (Novus, Monoclonal,
clone 13B8E5C2) Company Recommended
Dilution: 1:5000. Mouse Colon Section,
Paraffin embedded.
(YES, 1 IN 5 MILLION!!) (Novus, Monoclonal,
clone 13B8E5C2) Company Recommended
Dilution: 1:5000. Mouse Colon Section,
Paraffin embedded.
1000 FOLD DILUTION
FROM COMPANY
RECOMMENDATION
FROM COMPANY
RECOMMENDATION
Anti-p53 1:1,000,000 (YES, 1 IN 1 MILLION!!)
(Exalpha, Biologicals, Inc. Monoclonal, mouse
clone Pab122, cat# X1494M). Company
recommended dilution: 1:1000. Mouse Colon
Section from mouse with colitis. Paraffin
embedded.
(Exalpha, Biologicals, Inc. Monoclonal, mouse
clone Pab122, cat# X1494M). Company
recommended dilution: 1:1000. Mouse Colon
Section from mouse with colitis. Paraffin
embedded.
Anti-iNOS 1:10,000 (Res & Diagnostic
Antibodies, Monoclonal Clone 5D5-H7, Cat#
MC-5245). Company Recommended Dilution:
1:50. Colon section from mouse with mild
colitis, paraffin embedded.
Antibodies, Monoclonal Clone 5D5-H7, Cat#
MC-5245). Company Recommended Dilution:
1:50. Colon section from mouse with mild
colitis, paraffin embedded.
Anti-Phospho-Serine-15 53 1:20,000 (Mouse monoclonal, Cell Signaling, Clone 16G8, cat#9286S).
Company recommended dilution: 1:100. A. Human Colon Cancer; B. Mouse Colitis Section. Paraffin
embedded
Company recommended dilution: 1:100. A. Human Colon Cancer; B. Mouse Colitis Section. Paraffin
embedded
200 FOLD DILUTION
FROM COMPANY
RECOMMENDATION
FROM COMPANY
RECOMMENDATION
CONTACT
Phone: 803-407-0506
Fax: 803-407-0260
Email: prohisto@yahoo.com
Phone: 803-407-0506
Fax: 803-407-0260
Email: prohisto@yahoo.com
Anti-AKT 1:5,000 (Cell Signaling Technologies.
Polyclonal, Rabbit cat# 9272). Company
recommended dilution: 1:50. Mouse Colon
Section from normal mouse. Paraffin
embedded.
Polyclonal, Rabbit cat# 9272). Company
recommended dilution: 1:50. Mouse Colon
Section from normal mouse. Paraffin
embedded.
100 FOLD DILUTION
FROM COMPANY
RECOMMENDATION
FROM COMPANY
RECOMMENDATION
1000 FOLD DILUTION
FROM COMPANY
RECOMMENDATION
FROM COMPANY
RECOMMENDATION
13,333 FOLD
DILUTION FROM
COMPANY
RECOMMENDATION
DILUTION FROM
COMPANY
RECOMMENDATION
200 FOLD DILUTION
FROM COMPANY
RECOMMENDATION
FROM COMPANY
RECOMMENDATION
PRODUCT INSERT DOES
NOT SAY IT CAN BE USED
WITH PARAFIN EMBEDDED
TISSUE SECTIONS
NOT SAY IT CAN BE USED
WITH PARAFIN EMBEDDED
TISSUE SECTIONS
50 FOLD DILUTION
FROM COMPANY
RECOMMENDATION
FROM COMPANY
RECOMMENDATION
Anti P21WAF1 1:2000 (Dako, Monoclonal,
SX108). Company Recommended Dilution:
1:25. Mouse Thymus Section, paraffin
embedded.
SX108). Company Recommended Dilution:
1:25. Mouse Thymus Section, paraffin
embedded.
80 FOLD DILUTION
FROM COMPANY
RECOMMENDATION
FROM COMPANY
RECOMMENDATION
Anti-p44/p42 MAP Kinase 1:1,000 (Cell
Signaling Technologies, Polyclonal,
Cat#9102). Company Recommended
Dilution: 1:25. Human breast cancer
section.
Signaling Technologies, Polyclonal,
Cat#9102). Company Recommended
Dilution: 1:25. Human breast cancer
section.
40 FOLD DILUTION
FROM COMPANY
RECOMMENDATION
FROM COMPANY
RECOMMENDATION
Anti-ERalpha 1:20,000 (Neomarkers) Polyclonal,
rabbit cat# RB-1493-P0). Company recommended
dilution: 1:500. Human Breast Cancer Section.
Paraffin embedded.
rabbit cat# RB-1493-P0). Company recommended
dilution: 1:500. Human Breast Cancer Section.
Paraffin embedded.
40 FOLD DILUTION
FROM COMPANY
RECOMMENDATION
FROM COMPANY
RECOMMENDATION