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Here is a continued selection of antibodies we have tested using
the Antibody Amplifier™.  Some have never worked, or worked
poorly, using the traditional sedentary ("dropping on") method.
We will add new antibodies to this site as they are tested. If your lab has
success with a particular antibody using the Antibody Amplifier,
we invite you to send a picture and we will add it to our website.
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FOR A DEMO, SEE:
DEMONSTRATION
IMMUNOFLOURESCENCE
In addition to IHC, the Antibody Amplifier™ can be used for  immunoflourescence. It's sturdy
construction and snap-on lid make the transportation of slides from hood to benchtop to microscope
easy and eliminates the worry of reagent run-off. Again, because the slides are immersed in dye
solution instead of "dropping on", your confidence in final results is greatly increased.
A ) THIS IS THE DETECTION OF REACTIVE OXYGEN SPECIES IN STIMULATED LIVE MOUSE
MACROPHAGES.  THE GREEN COLOR IS THE FLOURESCENT DYE
5-CARBOXY-2'-7'-DICHLORODIHYDROFLOURESCEIN DIACETATE.
B) THESE ARE THE ABOVE LIVE MOUSE MACROPHAGES, STAINED WITH THE NUCLEIC
ACID DYE, HOECHST 33342;
BACK
Comparison of
Sedentary vs. Antibody
Amplifier Method.
Anti-Ki67 1:1,000,000
(Dako, Monoclonal,
MIB-1). Human tonsil
serial sections. Using the
standard sedentary
method, the antibody
does not work at this
dilution
Anti-Fas-L 2.5 ug/ml (Calbiochem,
polyclonal, Cat#PC78). Recommended
Dilution for sedentary method: Not
recommended for IHC. Mouse thymus
section, paraffin embedded.
Anti-TRAIL 1:5000 (R&D Systems,
Polyclonal, Cat#BAF1121). Recommended
Dilution for sedentary method: 1:25.
Mouse spleen section, paraffin embedded.
A
B
Anti-p53 Pab122, diluted at 1:1,000,000 using a greatly diluted Alexa Fluor 568
labeled secondary antibody.  Mouse colon from mouse with colitis
CONTACT
Phone: 803-407-0506
Fax: 803-407-0260
Email: prohisto@yahoo.com
200 FOLD DILUTION
FROM SEDENTARY
METHOD
COMPANY DOES  
NOT RECOMMEND
FOR IHC
Anti-Bcl-2 1:5000 (Cell Signaling,
Polyclonal, Cat#2876). Recommended
Dilution for sedentary method: Not
Recommended for IHC. Mouse colitis
section, paraffin embedded.
COMPANY DOES  
NOT RECOMMEND
FOR IHC
Anti-Bax 1:3000 (Cell Signaling,
Polyclonal, Cat#2772). Recommended
Dilution for sedentary method: Not
Recommended for IHC. Mouse colitis
section, paraffin embedded.
COMPANY DOES  
NOT RECOMMEND
FOR IHC
Anti-PUMA 1:7500 (Cell Signaling,
Polyclonal, Cat#4976). Recommended
Dilution for sedentary method: 1:200.
Mouse colon section, paraffin
embedded.
20 FOLD DILUTION
FROM SEDENTARY
METHOD
Anti-PolB 1:1000 (Abcam, Polyclonal,
Cat#AB53059). Recommended Dilution
for sedentary method: 1:50. Mouse
tumor section, paraffin embedded.
38 FOLD DILUTED
FROM SEDENTARY
METHOD
NEXT
Anti-TNF-alpha 1:50,000
(Abcam, monoclonal,
Cat#9579). Recommended
Dilution for sedentary
method: 1:100. Mouse
colitis and normal colon
section, paraffin
embedded.
500 FOLD DILUTED
FROM SEDENTARY
METHOD
Anti-IFN-gamma 1:100,000
(Lifespan Biosciences,
polyclonal, Cat#LS-C7639).
Recommended Dilution for
sedentary method: 1:500.
Mouse colitis section, paraffin
embedded.
200 FOLD DILUTED
FROM SEDENTARY
METHOD
NEXT
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