Inappropriate protein content in secondary antibody buffers can drastically affect the performance of labeled secondary antibodies. ProHisto's Universal Secondary Antibody Buffer contains only the ingredients neccessary to boost your secondary antibody's performance, whether it is enzyme or flourescent labeled. Each bottle of Universal Secondary Antibody Buffer is enough for 160 slides; Although it contains a broad spectrum anti-microbial, it has no perservatives to interfere with key enzymes such as peroxidases.