Here is a selection of antibodies we have tested using the Antibody Amplifier™.
Some have never worked, or worked poorly, using the traditional sedentary ('dropping on')
method. We will add new antibodies to this site as they are tested.
Should you have any questions regarding any of the ProHisto products, please contact us at info@prohisto.com.
Click Here for our Protocols.
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Antibody |
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| Anti-acetylated lysine, 1:100,000 (Stressmarq
Biosciences, Inc. monoclonal Cat# SMC-153). Recommended Dilution for sedentary method: Not Determined. Human GI tissue, paraffin embedded. |
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| Anti-AHA-1, 1:100,000 (Stressmarq Biosciences,
Inc. polyclonal). Recommended Dilution for sedentary method: Not Determined. Mouse colitis section, paraffin embedded. |
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| Anti-AHA-1, 1:100,000 (Stressmarq Biosciences,
Inc. polyclonal). Recommended Dilution for sedentary method: Not Determined. Human GI tissue, paraffin embedded. |
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| Anti-AKT 1:5,000 (Cell Signaling Technologies. Polyclonal,
Rabbit cat# 9272). Recommended dilution for sedentary
method: 1:50. Mouse Colon Section from normal mouse. Paraffin embedded. |
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100 FOLD DILUTION FROM SEDENTARY METHOD |
| Anti-AP endonuclease 1 (APE1) 1:5,000,000 (YES, 1 IN 5 MILLION!!) (Novus, Monoclonal, clone 13B8E5C2) Recommended Dilution for sedentary method: 1:5000. Mouse Colon Section, Paraffin embedded. |  |
1000 FOLD DILUTION FROM SEDENTARY METHOD |
| Anti-Bax 1:3000 (Cell Signaling, Polyclonal, Cat#2772). Recommended Dilution for sedentary method: Not Recommended for IHC. Mouse colitis section, paraffin embedded. |  |
The Manufacturer does not recommend for IHC. |
| Anti-Bcl-2 1:5000 (Cell Signaling, Polyclonal, Cat#2876). Recommended Dilution for sedentary method: Not Recommended for IHC. Mouse colitis section, paraffin embedded. |  |
The Manufacturer does not recommend for IHC. |
| Anti-Calnexin-CT, 1:100,000 (Stressmarq
Biosciences, Inc. Polyclonal, Cat# SPC-200). Mouse colitis, paraffin embedded. |
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| Anti-Calnexin-NT, 1:10,000 (Stressmarq Biosciences,
Inc. Polyclonal, Cat# SPC-127). Recommended
Dilution for sedentary method: Not Determined.
Mouse Colon, paraffin embedded. |
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| Anti-Calreticulin, 1:10,000 (Stressmarq Cat# SC-122). Recommended Dilution for sedentary method: Not Determined. Mouse colitis section, paraffin embedded. |  |
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| Anti-CD4, 1:20,000 (Lifespan Biosciences,
Monoclonal, clone 2Q1171, Cat# LS-C4476).
Recommended Dilution for sedentary method: Not Determined. Mouse colitis section, paraffin embedded. |
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| Anti-CD8, 1:100,000 (Abcam, Rabbit Monoclonal,
clone EP1150Y, Cat# ab52854). Recommended Dilution for sedentary method: 1:250. Mouse colitis section, paraffin embedded. |
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400 FOLD DILUTION FROM SEDENTARY METHOD |
| Anti-CD74, 1:10,000 (Stressmarq Cat# SPC-116).
Recommended Dilution for sedentary method:
Not Determined. Human colon cancer, paraffin
embedded. |
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| Anti-COX2 1:20,000 (Cayman, Polyclonal, Cat#160126) Recommended Dilution for sedentary method: 1:400. Colon section from mouse with colitis, paraffin embedded. |  |
50 FOLD DILUTION FROM SEDENTARY METHOD |
| Anti-Crystallin alpha B, 1:10,000 (Stressmarq
Biosciences, Inc. Polyclonal, Cat# SPC-126). Recommended Dilution for sedentary method: Not Determined. Mouse colitis section, paraffin embedded. |
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| Anti-DNA/RNA Damage 1:10,000 (Stressmarq Biosciences, Inc., Mouse Monoclonal, clone 15A3, cat# SMC-155) (8-hydroxy-guanosine-BSA and –casein conjugates). Recommended dilution for sedentary method: 1:130. Mouse Colon Section from mouse with colitis. Paraffin embedded. |  |
770 FOLD DILUTION FROM SEDENTARY METHOD |
| Anti-ERalpha 1:20,000 (Neomarkers) Polyclonal, rabbit
cat# RB-1493-P0). Recommended dilution for sedentary
method: 1:500. Human Breast Cancer Section. Paraffin
embedded. |
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40 FOLD DILUTION FROM SEDENTARY METHOD |
| Anti-Fas-L 2.5 ug/ml (Calbiochem, polyclonal,
Cat#PC78). Recommended Dilution for
sedentary method: Not recommended for IHC.
Mouse thymus section, paraffin embedded. |
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The Manufacturer does not recommend for IHC. |
| Anti-FKBP51, 1:10,000 (Stressmarq Biosciences,
Inc., monoclonal Cat# SMC-139). Recommended
Dilution for sedentary method: Not determined. Human breast carcinoma, paraffin embedded. |
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| Anti-FOXP3, 1:100,000 (BioLegend, Monoclonal,
clone 150D, Cat# 320001). Recommended
Dilution for sedentary method: 1:50. Mouse
colitis section, paraffin embedded. |
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2000 FOLD DILUTION FROM SEDENTARY METHOD |